Enhancement of migration and invasion of hepatoma cells via a Rho GTPase signaling pathway

Figure 3 Inhibition of LPA-induced tumor cell invasion by Y-27632. Serum-starved cells were seeded onto porous filters. After incubation of the filters with Y-27632 in the presence of LPA 25 μM for 4.5 h to permit penetration of the cells, nonmigrating cells were removed from the upper chamber and migrating cells adherent to the underside of the filters were counted in a minimum of 10-high power fields. Data were expressed as relative migration (number of cells/field) and represented as ^-x±s from triplicate experiments. ^aP < 0.01 relative to SMMC-7721 control cells.