Construction of prokaryotic expression system of ltB-ureB fusion gene and identification of the recombinant protein immunity and adjuvanticity

Figure 1 Target amplification fragments of ltB and ureB genes and ltB-ureB fusion gene. Lane 1: 250 bp DNA marker (BBST); Lanes 2, 4 and 6: Blank controls; Lanes 3 and 5: Target amplification fragments of ltB gene and ltB-ureB fusion gene, respectively; Lane 7: Target recovered fragment of ureB gene from pUCm-T-ureB after digestion with both EcoR V and Xho I.