Brief Reports
Copyright ©The Author(s) 2004.
World J Gastroenterol. Sep 1, 2004; 10(17): 2560-2562
Published online Sep 1, 2004. doi: 10.3748/wjg.v10.i17.2560
Figure 3
Figure 3 The 100 g/L SDS-PAGE analysis of fusion protein expressed in BL21 (DE3). Lane 1: Molecular mass marker (67, 94) × 103; Lane 2: control strain BL21 (pET) before induction; Lane 3: control strain BL21 (pET) after 5-h induction with IPTG; Lane 4: BL21 (pET-BabA) cells before induction; Lane 5: BL21 (pET- BabA) cells after 3-h induction with IPTG; Lane 6: BL21 (pET- BabA) cells after 5-h induction with IPTG; Lane 7: BL21 (pET- BabA) cells periplasm protein after 3-h induction with IPTG; Lane 8: sonicated supernatant of BL21 (pET-BabA) cells after 3-h induction with IPTG; Lane 9: recombinant protein BabA purified.