Helicobacter pylori cagA, iceA and vacA genotypes in patients with gastric cancer in Taiwan

Table 1 Oligonucleotide primers used for cagA, vacA and iceA genotyping

Region detected	Primer designation	Primer sequence	Size of PCR product (bp)	References
s1 and s2	VA1-F	5’ATGGAAATACAACAAACACACC3’	259/286	14
	VA1-R	5’CTGCTTGAATGCGCCAAACTTTATC3’
s1a	SS1-F	5’GTCAGCATCACACCGCAAC3’	190	20
s1b	SS3-F	5’AGCGCCATACCGCAAGAG3’	187	20
s1c	S1C-F	5’CTYGCTTTAGTRGGGYTA3’	213	26
M1	VA3-F	5’GGTCAAAATGCGGTCATGG3’	290	20
	VA3-R	5’CCATTGGTACCTGTAGAAAC3’
M1T	m1T-F	5’GGTCAAAATGCGGTCATGG3’	290	14
	m1T-R	5’CTCTTAGTGCCTAAAGAAACA3’
M2	VA4-F	5’GGAGCCCCAGGAAACATTG3’	352	20
	VA4-R	5’CATAACTAGCGCCTTGCAC3’
iceA1	iceA1F	5’GTGTTTTTAACCAAAGTATC3’	247	21
	iceA1R	5’CTATAGCCASTYTCTTTGCA3’
iceA2	iceA2F	5’GTTGGGTATATCACAATTTAT3’	229	21
	iceA2R	5’TTRCCCTATTTTCTAGTAGGT3’
lcagA	lcagAD008	5’ATAATGCTAAATTAGACAACTTGAGCGA3’	297	8
	lcagAR008	5’TTAGAATAATCAACAAACATCACGCCAT3’