Simultaneous detection of HBV and HCV by multiplex PCR normalization

Table 2 Amplification results of HCV RNA from 15 anti-HCV positive sera

Method	Sample number
	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15
ELISA	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
Method 1	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-
Method 2	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-
Method 3	-	+	+	+	-	-	-	-	+	+	-	-	+	-	-
Mrthod 4	+	+	+	+	+	+	+	+	+	+	-	+	+	+	+

Note: + represents positive amplification; - represents negative amplification.