HCV NS5A abrogates p53 protein function by interfering with p53-DNA binding

Table 1 HCV NS5A inhibits the transactivation of endogenous p53 on p21 promoter

Group	Relative luciferase activity	F	P
pwwp-luc (1 µg)
+ pCNS5A (0 µg)	3.49 × 105b	20.71	< 0.01
+ pCNS5A (0.5 µg)	2.69 × 105
+ pCNS5A (1.0 µg)	1.62 × 105
+ pCNS5A (2.0 µg)	0.58 × 105
pwwp-mut-luc (1.0 μg)
+ pCNS5A (0 µg)	0.60 × 105	0.76	> 0.05
+ pCNS5A (0.5 µg)	0.65 × 105
+ pCNS5A (1.0 µg)	0.62 × 105
+ pCNS5A (2.0 µg)	0.64 × 105

^bt = 5.92, P < 0.01.