A rapid and efficient method to express target genes in mammalian cells by baculovirus

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Jun 1, 2004 (publication date) through May 7, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jun 1, 2004; 10(11): 1612-1618
Published online Jun 1, 2004. doi: 10.3748/wjg.v10.i11.1612

Table 2 Comparison of gene-transfer and expression efficiencies in different mammalian cell lines transfected with pcDNA3.1-EGFP

Organism		Tissue	Growth properties	Transfection efficiency (%)	Mean fluorescence intensity
Human
	WI-38	Lung fibroblast	Adherent	24.53	58.3
	HepG2	Hepatocellular carcinoma	Adherent	18.60	79.4
	Hela	Adenocarcinoma	Adherent	19.82	42.7
	293	Kidney	Adherent	61.45	86.6
	Raji	Burkitt’s lymphoma	Suspension	5.56	14.6
	LCL-cm	B lymphocyte	Suspension	7.62	17.4
Mouse
	BNL 1ME A.7R.1	Liver	Adherent	13.09	19.1
	CHO-K1	Ovary	Adherent	47.36	48.6
	JC	Adenocarcinoma	Adherent	12.54	22.8
	PT67	Embryo fibroblast	Adherent	22.63	31.6
	NIH3T3	Embryo fibroblast	Adherent	25.31	35.3
	P815	Mastocytoma	Suspension	12.78	16.8

Citation: Cheng T, Xu CY, Wang YB, Chen M, Wu T, Zhang J, Xia NS. A rapid and efficient method to express target genes in mammalian cells by baculovirus. World J Gastroenterol 2004; 10(11): 1612-1618
URL: https://www.wjgnet.com/1007-9327/full/v10/i11/1612.htm
DOI: https://dx.doi.org/10.3748/wjg.v10.i11.1612