Construction and identification of recombinant vectors carrying herpes simplex virus thymidine kinase and cytokine genes expressed in gastric carcinoma cell line SGC7901

Figure 2 Segment analyses of pEGFP-TI and pEGFP-TT by restriction endonuclease digestion. Lane 1: DNA marker (from top to bottom: 1 543, 994, 697, 515 and 337 bp); Lane 2: pEGFP-TI/EcoR I and Hpa I (TK gene 1 128 bp); Lane 3: pEGFP-TI/ EcoRI and XhoI (TK + IRES 1.71kb); Lane 4: pEGFP-TI/XhoI and BamH I (IL-2 gene 456 bp); Lane 5: pEGFP-TT/EcoRI and HpaI (TK gene 1 128 bp); Lane 6: pEGFP-TT/EcoRI and XhoI (TK + IRES 1.71 kb); Lane 7: pEGFP-TT/XhoI and BamHI (TNF-α gene 474 bp).