蟾毒灵对人肝癌细胞增殖与侵袭的影响

表1 不同浓度和作用时间蟾毒灵对肝癌BEL-7402细胞增殖的抑制率 (n = 3, mean±SD)

浓度(µg/mL)	24 h	48 h	72 h
0.05		22.09±3.63d	35.52±4.16f
0.10		33.94±3.34	53.17±8.65
0.15			59.21±8.11
0.20		47.72±2.24	78.88±3.93
0.25			86.86±1.54f
0.30		68.84±1.98
0.40		82.57±1.09d
0.70	31.34±6.73b
0.80	48.05±8.52
0.90	51.62±5.79
1.00	60.38±5.69
1.10	70.97±5.19
1.20	72.98±4.15b

^bF = 40.624, ^bP<0.01 vs 0.90 µg/mL;

^dF = 427.344, ^dP<0.01 vs 0.20 µg/mL;

^fF = 69.585, ^fP<0.01 vs 0.15 µg/mL.

表2 同一浓度不同作用时间蟾毒灵对肝癌BEL-7402细胞增殖周期的影响 (n = 3, mean±SD, %)

周期	24 h		48 h		72 h
	阴性对照	蟾毒灵	阴性对照	蟾毒灵	阴性对照	蟾毒灵
G1期	48.26±5.09	48.34±2.72	62.20±3.48	41.2±1.27	68.49±2.48	31.98±1.52
G2期	28.98±3.67	29.78±2.14	9.28±2.06	19.78±1.82b	14.27±1.49	31.75±1.51b
S期	22.76±3.68	21.88±0.60	28.53±1.49	39.02±3.06b	17.24±3.66	34.94±5.22b

^bP<0.01 vs 阴性对照[48 h(G₂: t = -6.618; S: t = -5.339), 72 h(G₂: t = -14.273; S: t = -4.812)].