大肠癌
Copyright ©The Author(s) 2004.
世界华人消化杂志. 2004-08-15; 12(8): 1800-1805
在线出版 2004-08-15. doi: 10.11569/wcjd.v12.i8.1800
表1 Differential Screening 杂交结果判断标准
探针类型
杂交结果分析
AR1-C2-C
+-+-阳性差异表达结果
+---阳性差异表达基因(强烈提示低丰度表达差异基因)
+-++多为假阳性结果,但是也可能模板与不同长度地探针杂交造成结果难以判断,多为多基因家族成分
++++±绝少情况为差异表达基因
++++非阳性差异表达基因
----非阳性差异表达基因
表2 11个差异片段与人已知基因的同源性
Clone(bp)Known gene descriptionAcc1essionIdentitiesExpression
A80(400)Heat Shock 10kDNM_002157200/209 (95%) in sw620Protein1 (HSP10)
A98(138)Cytochrome CNM_17370575/75(100%) in sw620OxidaseII (COXII)
A108(181)Mitotic Control ProteinNM_014953130/131(99%) in sw620dis3 homolog(DIS3)
A164(300)skeletal muscle actinNM_001100167/168(99%) in sw620alpha 1 (ACTA1)
A184(301)Serum amyloid A(SAA)NM_030754276/276 (100%) in sw620
R159(91)egl nine homolog 2NM_08073290/91(98%) in sw480(EGLN2)
R57(450)Eukaryotic TranslationNM_001416448/450(99%) in sw480Initiation Factor 4A(ETIF4A)
R178(298)Glutathione S-transferaseAF043105260/264(98%) in sw480mu 3 (GSTM3)
R211(155)Similar to Cytochrome cBC013930153/155(98%) in sw480Oxidase III(COXIII)
R32(214)Haplotype As9yAY195792213/213(100%) in sw480mitochondrion

引文著录: 梁莉, 丁彦青, 李欣, 杨玉芳, 肖军, 张进华, 赵培冉. 人大肠癌转移相关基因片段的筛选和鉴定. 世界华人消化杂志 2004; 12(8): 1800-1805