鼠肝炎病毒3型N蛋白I区激活 mfgl2凝血酶原酶基因

表1 MHV-A59, MHV-3, MHV-2和MHV-JHM的N蛋白氨基酸序列比较

		* G12S	* P38 del		P38L *	* NQN 40-42 del
MHV-A59	1	MSFVPGQENA	GGRSSSVNRA	GNGILKKTTW	ADQTERGPNN	QNRGRRNQPK	50
MHV-3		G	G
MHV-2		S	G		- - - -	H
MHV-JHM		S	G		L	K
					*E85Q
MHV-A59	51	QTATTQPNSG	SVVPHYSWFS	GITQFQKGKE	FQFAEGQGVP	IANGIPASEQ	100
MHV-3					E
MHV-2		A			Q	S
MHV-JHM					Q	Q
MHV-A59	101	KGYWYRHNRR	SFKTPDGQQK	QLLPRWYFYY	LGTGPHAGAS	YGDSIEGVFW	150
MHV-3					S
MHV-2					E
MHV- JHM			H		E
MHV-A59	151	VANSQADTNT	RSDIVERDPS	SHEAIPTRFA	PGTVLPQGFY	VEGSGRSAPA	200
MHV-3		NS	S
MHV-2		SQ K	TA V	K		K
MHV-JHM		SQ E R	SA
MHV-A59	201	SRSGSRSQSR	GPNNRARSSS	NQRQPASTVK	PDMAEEIAAL	VLAKLGKDAG	250
MHV-3				A
MHV-2
MHV-JHM		P
MHV-A59	251	QPKQVTKQSA	KEVRQKILNK	PRQKRTPNKQ	CPVQQCFGKR	GPNQNFGGSE	300
MHV-3
MHV-2			T
MHV-JHM						P
				*V321A
MHV-A59	301	MLKLGTSDPQ	FPILAELAPT	VGAFFFGSKL	ELVKKNSGGA	DEPTKDVYE	350
MHV-3
MHV-2				PS
MHV-JHM				A		G
MHV-A59	351	QYSGAVRFDS	TLPGFETIMK	VLNENLNAYQ	K-DGGADVVS	PKPQRKGRRQ	400
MHV-3					_
MHV-2		I		T	DQA SV L	P R
MHV-JHM					NQ	RGTK
MHV-A59	401	AQEKKDEVDN	VSVAKPKSSV	QRNVSRELTP	EDRSLLAQIL	DDGVVPDGLE	450
MHV-3
MHV-2			L
MHV-JHM		QKAQ
MHV-A59	451	DDSNV* .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. ..
500 MHV-3
MHV-2
MHV-JHM

表2 MHV-A59 N蛋白氨基酸突变位点和引物序列

Mutant Name	Mutation	Location of Mutation	Sense Primer (5'-3')/Antisense Primer (5'-3')
A59G12S	Gly12 to Ser12	Domain I	CCT GGG CAA GAA AAT GCC GGT AGC AGA AGC TCC TCT G/
			C AGA GGA GCT TCT GCT ACC GGC ATT TTC TTG CCC AGG
A59P38L	Pro38 to Leu38	Domain I	GAC CAA ACC GAG CGT GGA CTA AAT AAT CAA AAT AGA GGC/
			GCC TCT ATT TTG ATT ATT TAG TCC ACG CTC GGT TTG GTC
A59P38del	Pro38 deletion	Domain I	GCT GAC CAA CAA ACC GAG CGT GGA CCA AAT AAT CAA AAT AGA GGC AGA AGG/
			CCT TCT GCC TCT ATT TTG ATT ATT TGG TCC ACG CTC GGT TTG TTG GTC AGC
A59NQN	Asn40-Gln41-	Domain I	GG GCT GAC CAA ACC GAG CGT GGA CCA AAT (AAT CAA AAT) AGA GGC AGA AGG
40-42del	Asn42 deletion		AAT CAG CCA AAG CAG ACT GC
			GC AGT CTG CTT TGG CTG ATT CCT TCT TCC TCT (ATT TTG ATT) ATT TGG TCC ACG
			CTC GGT TTG TTC AGC CC
A59E85Q	Glu85 to Gln85	Domain I	GGA AAG GAG TTT CAG TTT GCA CAG GGA CCA GGA GTG CCT ATT GCC
			GGC AAT AGG CAC TCC TGG TCC CTG AGC AAA CTG AAA CTC CTT TCC
A59V321A	Val321 to Ala321	Domain II	GCA GAG TTG GCT CCA ACA CCT GGT GCC GGT GCC TTC TTC GG
			CC GAA GAA GGC ACC GGC ACC AGG TGT TGG AGC CAA CTC TGC

黑体字母表示突变序列. 括号内黑体字母表示缺失序列. 所有突变的产物皆经测序核实方向和序列.