幽门螺杆菌vacA毒性片段与hpaA融合基因的原核表达

图1 带有接头的v基因片段的PCR扩增. 1: DNA marker; 2: PCR product; 3: control.

图2 重组质粒pTrc99A-V-HpaA限制性酶切分析. 1: pTrc99A-HpaA /Ncoa+EcoRa; 2: pTrc99A-V-HpaA /Ncoa+ BamHⅠ; 3: pTrc99A-V-HpaA / Ncoa+EcoRa; 4: DNA marker.

图3 融合基因v-hpaA 的PCR扩增. 1: DNA marker; 2-4: PCR product; 5: control.

图4 重组质粒pQE-30-hpa-vacA限制性酶切分析. 1: pQE30-V-HpaA / BamHⅠ+ SalI; 2: pQE30-V-HpaA / EcoRa+ BamHI; 3: pQE30 / BamHⅠ+ SalI; 4; DNA marker.

图5 DH5a/pQE30-V-HpaA表达产物SDS-PAGE鉴定结果. 1: protein marker; 2: DH5a/pQE30 induced with IPTG; 3: DH5a/ pQE30-V-HpaA induced without IPTG; 4-8: DH5a/pQE30-V-HpaA induced with IPTG.

图6 融合蛋白的Western blotting鉴定结果. 1: Protein marker; 2: Noninduced protein exhibited with anti-serum against H. pylori Ab-HRP; 3-4: induced protein exhibited with anti-serum against H. pylori Ab-HRP.