幽门螺杆菌
Copyright ©The Author(s) 2003.
世界华人消化杂志. 2003-10-15; 11(10): 1475-1479
在线出版 2003-10-15. doi: 10.11569/wcjd.v11.i10.1475
图1
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图1 琼脂糖凝胶电泳分析PCR产物. 1 ltB PCR product; 2 hspA PCR product; 3 PCR marker.
图2
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图2 重组质粒pPLH的双酶切鉴定图谱. Marker; 1. Positive recombinant plasmid pPLH PstI+EcoRV; 2. Positive recombinant plasmid pPLH PstI+BamHI.
图3
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图3 重组质粒pPLH的构建示意图.
图4
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图4 ltB-HspA融合基因的核苷酸序列及推定的氨基酸序列.
图5
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图5 重组质粒pPLH表达产物SDS-PAGE分析. M: Molecular weight marker; 1: E.coli JM109/pPLH after induction with IPTG; 2: E.coli JM109/pPLH before induction; 3: E.coli JM109/PinPointTMXa-III after induction with IPTG; 4: E.coli JM109/PinPointTMXa-IIIbefore induction.
图6
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图6 重组LTB-HspA融合蛋白的免疫印迹分析. M: marker; 1: 诱导前重组菌; 2: 诱导5 h后重组菌.
引文著录: 郭红, 邹全明, 赵哓晏, 吴超. 幽门螺杆菌HspA与大肠杆菌LTB基因融合及表达. 世界华人消化杂志 2003; 11(10): 1475-1479