Novel deletion mutation in Bruton’s tyrosine kinase results in X-linked agammaglobulinemia: A case report

Figure 5 Western blot and relative translation analysis of E301 deletion mutant Bruton’s tyrosine kinase relative to wild-type Bruton’s tyrosine kinase. Markedly reduced protein band (A) and relative translation (B) were observed with the E301 deletion mutant relative to the wild-type gene. PEGFP-1 is the empty vector control group. Reduced glyceraldehyde-phosphate dehydrogenase was the loading control. Mean ± standard deviation levels are shown; ^aP < 0.001. BTK: Bruton’s tyrosine kinase; WT: Wild-type.