Cross electro-nape-acupuncture ameliorates cerebral hemorrhage-induced brain damage by inhibiting necroptosis

Figure 3 Cross electro-nape acupuncture inhibited cerebral hemorrhage -mediated necrosome formation in vivo. A and B: Propidium iodide staining (A) and propidium iodide positive rate (B) in the brain tissues of experimental rats; C: Protein level of RIPK1, RIPK3, p-RIPK3, MLKL and p-MLKL in the brain tissues of experimental rats; D-H: Quantitative analysis of RIPK1 (D), RIPK3 (E), p-RIPK3 (F), MLKL (G) and p-MLKL (H); I-K: RIPK1 and RIPK3 interaction in the brain tissues of experimental rats using IP assay (J) and quantitative analysis (K) of the IP assay. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. CENA: Cross electro-nape acupuncture; CH: cerebral hemorrhage; RIPK: Receptor interacting protein kinase; MLKL: mixed lineage kinase domain like pseudokinase.