In vitro differentiation of human umbilical cord Wharton&rsquo;s jelly mesenchymal stromal cells to insulin producing clusters

doi:10.12998/wjcc.v3.i7.640

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World Journal of Clinical Cases

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2307-8960

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Clin Cases. Jul 16, 2015; 3(7): 640-649
Published online Jul 16, 2015. doi: 10.12998/wjcc.v3.i7.640

Table 1 Primer pairs for amplification of transcription factors

Names	Forward (F) 5´-3´	Reverse(R) 5´-3´
Insulin	GCAGCCTTTGTGAACCAACA	TTCCCCGCACACTAGGTAGAGA
PDX1	GGATGAAGTCTACCAAAGCTCACGC	CCAGATCTTGATGTGTCTCTCGGTC
NGN3	CAATCGAATGCACAACCTCA	GGGAGACTGGGGAGTAGAGG
GLUT2	AGGACTTCTGTGGACCTTATGTG	GTTCATGTCAAAAAGCAGGG
NANOG	CAGAAGGCCTCAGCACCTAC	ATTGTTCCAGGTCTGGTTGC
Oct-4	CAGTGCCCGAAACCCACAC	GGAGACCCAGCAGCCTCAAA
β-actin	GATCGGCGGCTCCATCCTG	GACTCGTCATACTCCTGCTTGC

Citation: Nekoei SM, Azarpira N, Sadeghi L, Kamalifar S. In vitro differentiation of human umbilical cord Wharton’s jelly mesenchymal stromal cells to insulin producing clusters. World J Clin Cases 2015; 3(7): 640-649
URL: https://www.wjgnet.com/2307-8960/full/v3/i7/640.htm
DOI: https://dx.doi.org/10.12998/wjcc.v3.i7.640