Genetic investigation of the ubiquitin-protein ligase E3A gene as putative target in Angelman syndrome

Table 2 Primer pairs used for single strand conformation polymorphism

Exon and primers	Forward and reverse primers (5’->3’)	Region	Annealing temperature (℃)
7
Ex7F	GCC ACC TGA TCT GAC CAC T	Intron	52
Ex7R	GCA GTC TAG GGC AAC TCA AA	Intron
8
Ex8AF	GCC TTG ATG ATA TGT TGA GC	Intron	55
Ex8AR	AAT TCT AGC GCC TTT CTT GT	Exon
Ex8BF	GCC TGC ACG AAT GAG TTT TGT	Exon	55
Ex8BR	AGT TAT TAT TCC TGT CCG TTA CC	Intron
9
Ex9AF	TGT TTG GCT GTT TTA CTT TTA GA	Intron	55
Ex9AR	GGC ATC AAT ATC CAC AGA CAC A	Exon
Ex9BF	AGA AGC ATC TTC CTC AAG G	Exon	55
Ex9BR	CAC TTC CCC TCC CAC TAC	Exon
Ex9CF	CAA TGA ATT TAA CAG TCG A	Exon	55
Ex9CR	CAT CAT CTA TGA TAT GGT CAC G	Exon
Ex9DF	CGC ATG TAC AGT GAA CGA AGA A	Exon	55
Ex9DR	TGC ACA GGA ACA ACA AAA GTA T	Intron
10
Ex10F	GTT TGC TTT CTG TTT CCA TTT AC	Intron	52
Ex10R	ATC CTT CTT TTG CTG CTC TTC	Intron
11
Ex11F	CAA TGT TGC ATG CCT AAT TAC A	Intron
	GGT ACT TCG GTC AGA TTA AAA C	Intron
12
Ex12F	GGG GAC TGG AGG GAT ACT GT	Intron	55
	ACA TGC TTT GAA AGT GTT AAT G	Intron
13
Ex13F	GAA ATT GTT AAG AAG TAG GTG	Intron	52
Ex13R	ATA TGT CTT AGT TAT CTG CTA	Intron
14
Ex14F	AGG TGT CTG CAA AAA GTC	Intron	55
Ex14R	TTA GCT CTG AAA AAT GGT G	Intron
15
Ex15F	ATA ATG AAT GCC AAA CTG AA	Intron	55
Ex15R	ATA TGT ATG TGA CGA GGA ATG	Intron
16
Ex16F	CCC ATG ACT TAC AGT TTT CCT G	Intron	55
Ex16R	AAG AAG GGA GGC ACA GAC AT	Intron