Early embryonic failure caused by a novel mutation in the TUBB8 gene: A case report

Table 1 Description and characteristics of assisted reproductive technology cycles

	Cycle 1	Cycle 2	Cycle 3	Cycle 4	Cycle 5
COH protocol	GnRH-A Long	GnRH-Ant	Natural cycle	Luteal phase	Natural cycle
Gonadotropin	r-FSH	r-FSH	-	HMG	-
Initiation dose (IU)	200	225	-	225	-
Total dose of gonadotropin (IU)	1975	2025	-	2025	-
Duration of gonadotropin (d)	9	9	-	9	-
E2 level on the day of HCG injection	6605.24	5822.55	189.60	2239.37	172.30
LH level on the day of HCG injection	1.54	0.57	4.87	1.86	4.04
P level on the day of HCG injection	2.05	2.56	0.28	7.38	0.21
No. of follicles (≥ 18 mm)	3	6	0	4	0
No. of follicles (≥ 14 mm)	9	8	1	8	1
Ovulation trigger (dose)	6000 IU (HCG)	2000 IU (HCG) + 0.2 mg (GnRH agonist)	0.1 mg (GnRH agonist)	6000 IU (HCG)	0.1 mg (GnRH agonist)
Interval between HCG administration and oocyte retrieval (h)	35.5 h	35 h	35 h	35 h	34 h
Number of retrieved oocytes	12	15	1	12	1
Fertilization mode	R-ICSI	ICSI	ICSI	ICSI	-
No. of MII	8	13	0	11	0
No. of GV	2	1	0	0	-
No. of 2PN	5	10	-	8	-
No. of D2 zygote	5	10	-	8	-
No. of D3 embryos	5	7	-	6	-
No. of blastocysts	0	0	-	0	-
No. of transferred embryos	1 (FET)	-	-	-	-
Pregnancy outcome	Not pregnant	No transferable embryos	No transferable embryos	No transferable embryos	No transferable embryos

COH: Controlled ovarian hyperstimulation; MII: Metaphase II; MI: Metaphase I; GV: Germinal vesicle; 2PN: 2 pronucleus; FET: Frozen Embryo Transfer.