High expression of autophagy-related gene EIF4EBP1 could promote tamoxifen resistance and predict poor prognosis in breast cancer

Figure 4 Overexpression of eukaryotic translation initiation factor 4E binding protein 1 in T47DR cells increased the resistance to tamoxifen. A: In T47D-R cells that upregulated the expression of eukaryotic translation initiation factor 4E binding protein 1 (EIF4EBP1), the expression of EIF4EBP1 was detected by real-time reverse transcription polymerase chain reaction; B: Cell Counting Kit-8 assay of EIF4EBP1 overexpression in T47D-R cells treated with tamoxifen; C: Colony formation experiments of EIF4EBP1 overexpression in T47D-R cells treated with tamoxifen; D: The wound healing assay showed that upregulated expression of EIF4EBP1 can more effectively increase migration in the T47D-R cell line; E: The Transwell assay showed that upregulated expression of EIF4EBP1 can increase cell invasion in the T47D-R cell line. Data are expressed as the mean ± SD from three independent experiments. ^bP < 0.01. EIF4EBP1: Eukaryotic translation initiation factor 4E binding protein 1; NC: Negative control; ov: Overexpression.