Clinical and Translational Research
Copyright ©The Author(s) 2023.
World J Clin Cases. Jul 16, 2023; 11(20): 4788-4799
Published online Jul 16, 2023. doi: 10.12998/wjcc.v11.i20.4788
Figure 3
Figure 3 Knockdown of eukaryotic translation initiation factor 4E binding protein 1 in T47DR cells reduced the resistance to tamoxifen. A: In T47D-R cells that downregulated the expression of eukaryotic translation initiation factor 4E binding protein 1 (EIF4EBP1), the expression of EIF4EBP1 was detected by real-time reverse transcription PCR; B:Cell Counting Kit-8 assay of EIF4EBP1 knockdown in T47DR cells treated with tamoxifen; C: Colony formation experiments of EIF4EBP1 knockdown in T47DR cells treated with tamoxifen; D: Wound healing assay showed that downregulated expression of EIF4EBP1 can more effectively decrease migration in the T47D-R cell line; E: The Transwell assay showed that downregulated expression of EIF4EBP1 can inhibit the cell invasion in the T47D-R cell line. Data are expressed as the mean ± standard deviation from three independent experiments. bP < 0.01. EIF4EBP1: Eukaryotic translation initiation factor 4E binding protein 1; NC: Negative control; si: Silencing RNA.