Development of clustered regularly interspaced short palindromic repeats/CRISPR-associated technology for potential clinical applications

Table 3 Clustered regularly interspaced short palindromic repeats-based tumor diagnostic system

System	Cas	Tumor type	Sensitivity	Specificity	Ref.
RCH	Cas9	NSCLC	10-15 M	High	[40]
CUT	Cas9	Colorectal cancer	< 0.01%	High	[41]
SHERLOCK	Cas13a	NSCLC	0.1%	High	[26]
SHERLOCKv2	Cas13a, Cas12a, Csm6	NSCLC	8 × 10-21 M	High	[29]
DETECTR	Cas12a	HPV	10-18 M	High	[30]

RCH: RCA-CRISPR-split-HRP; NSCLC: Non-small cell lung cancer; HPV: Human papillomavirus; CUT: CRISPR-mediated Ultrasensitive Detection of Target DNA-PCR; SHERLOCK: Specific high-sensitivity enzymatic reporter unlocking; DETECTR: DNA endonuclease-targeted CRISPR trans reporter.