Novel m.4268T>C mutation in the mitochondrial tRNAIle gene is associated with hearing loss in two Chinese families

Figure 3 Northern blot analysis of mitochondrial tRNA. A: Equal amounts (2 μg) of total mitochondrial RNA from various cell lines were electrophoresed through a denaturing polyacrylamide gel, electroblotted and hybridized with DIG-labeled oligonucleotide probes; B: Quantification of mitochondrial tRNA levels. The calculations were based on three independent determinations of the tRNA content in each cell line and three determinations of the content in the reference tRNA marker in each cell line. The horizontal dashed lines represent the average value for each group.