Low-dose intralesional injection of 5-fluorouracil and triamcinolone reduces tissue resident memory T cells in chronic eczema

Figure 4  Histopathology and immunohistochemistry of localized lesions of chronic eczema to intralesional treatment with 5-FU+TA and TA before and 2 wk after treatment. (A) Representative histologic images of biopsy specimens from localized lesions of chronic eczema were collected before and at 2 wk after treatment. Tissue samples (n = 15 for each group) were stained with HE and antibodies against CD4, CD8, or CD103 to detect T-cell infiltration in the lesional skin. (B) CD4⁺, CD8⁺, and CD103⁺ T cell counts were quantified in high-power field images of lesions biopsied before and 2 wk after treatment. Blue points: 5-FU + TA group; Red points: TA group. ^aP < 0.05, ^bP < 0.01. 5-FU, 5-fluorouracil; TA, triamcinolone.