Pathogenicity of a currently circulating Chinese variant pseudorabies virus in pigs

Table 1 Outcome of infections with pseudorabies virus HN1201 strain in pigs

Group	Pig age (d)	Pig No.	Virus titer	Route	Euthanized
1	60	5	107	im	3/5
2	60	5	107	in	5/5
3	60	5	106	in	2/5
4	60	5	105	in	1/5
5	35	5	107	in	5/5
6	127	5	107	in	5/5

im: Intramuscular; in: Intranasal.

Table 2 Clinical manifestations of pseudorabies virus HN1201 and Fa infection

Groups	Respiratory symptom					Neurological symptom
	Vomit	Respiratory distress	Cough	Sneeze	Salivation	Circling	Posterior paralysis	Muscle tremors	Lay recumbent and paddle
	+	+	-	+	-	+	-	+	-
I (HN1201)	-	+	+	+	-	+	-	+	-
	+	+	+	+	-	-	+	+	+
	-	+	+	+	+	+	+	+	+
	+	+	+	+	+	+	+	+	+
	-	-	-	+	-	-	-	-	-
II (Fa strain)	-	-	-	+	-	-	-	-	-
	-	-	-	+	-	-	-	-	-
	-	-	-	+	-	-	-	-	-
	+	+	-	+	+	+	-	+	+

Each row represents one pig in the corresponding group.

Table 3 Virus antigen distribution and intensity in different organs of pseudorabies virus HN1201 or Fa strain by immunohistochemistry staining

Groups	Tonsil	Lung	Lymph nodes			Brain	Cerebellum	Spleen	Liver
			Mandibular	Superficial inguinal	Mesenteric
HN1201	3+	3+	3+	2+	2+	2+	1+	2+	2+
	3+	2+	2+	2+	2+	2+	1+	3+	-
	3+	3+	3+	2+	2+	1+	2+	3+	+
	3+	3+	++	3+	2+	1+	2+	2+	2+
	3+	3+	3+	2+	3+	2+	2+	3+	3+
Fa strain	-	+	-	-	-	-	-	-	-
	-	-	-	-	-	2+	2+	-	-
	-	-	-	-	-	2+	2+	-	-
	-	-	-	-	-	2+	2+	-	-
	-	-	-	-	-	2+	2+	-	-

The positive staining signals were interpreted as negative (-), low (1+), moderate (2+), or intense (3+), according to the intensity of staining. Each row represents one pig in the corresponding group.