Structural and nucleic acid binding properties of hepatitis delta virus small antigen

Figure 4 Nuclear magnetic resonance spectra of ¹⁵N labeled ∆60HDAg. A: ¹H-¹⁵N HSQC of ∆60HDAg recorded on a Bruker 600 MHz Avance II NMR instrument at 25 °C with the standard Bruker pulse sequence: hsqcetfpf3gpsi. Four thousand and ninety-eight data points in ¹H dimension and 256 increments in ¹⁵N dimension were acquired; B: ¹H and ¹⁵N heteronuclear NOE spectrum of ∆60HDAg, superimposed on the normal ¹H-¹⁵N HSQC spectrum (gray contours). Heteronuclear NOE spectrum was recorded with the Bruker pulse sequence: Hsqcnoef3gpsi. Positives and negatives are displayed in red and blue contours, respectively. Peaks from glycines (boxed and labeled as 1 and 2 in the top of the spectra) are grouped.