Expression of hepatitis B virus surface antigens induces defective gonad phenotypes in Caenorhabditis elegans

Table 1 Comparison of egg-laying ability and brood size among various transgenic worms

Construct	Strain	Ecotopic proteins	Protein expression site	Egg-laying ability (%)	Brood size
	N2			100 (n = 17)	290 ± 15 (n = 17)
Pfib-1::gfp::icr	N2	GFP	Whole worm	100 (n = 10)	268 ± 29 (n = 18)
Pfib-1::gfp::icr::SHBsAg	N2	GFP, SHBsAg	Whole worm	9 (n = 60)	66 ± 15 (n = 14)
Pfib-1::gfp::icr::MHBsAg	N2	GFP, MHBsAg	Whole worm	10 (n = 60)	175 ± 50 (n = 15)
Pfib-1::gfp::icr::LHBsAg	N2	GFP, LHBsAg	Whole worm	54 (n = 60)	239 ± 14 (n = 15)
Pmyo-2::gfp::icr::SHBsAg	N2	GFP, SHBsAg	Pharynx	83 (n = 60)	163 ± 20 (n = 26)
Pges-1::gfp::icr::SHBsAg	N2	GFP, SHBsAg	Intestine	97 (n = 60)	203 ± 50 (n = 32)
Pmec-7::gfp::icr::SHBsAg	N2	GFP, SHBsAg	Neuron	100 (n = 60)	270 ± 42 (n = 26)

HBsAgs: Hepatitis B virus antigens; GFP: Green fluorescence proteins; SHBsAg: Human hepatitis B virus small surface antigens; MHBsAg: Human hepatitis B virus middle surface antigens; LHBsAg: Human hepatitis B virus large surface antigens.