Rapid genotyping of human rotavirus using SYBR green real-time reverse transcription-polymerase chain reaction with melting curve analysis

doi:10.5501/wjv.v4.i4.365

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Nov 12, 2015 (publication date) through Apr 24, 2024

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World Journal of Virology

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2220-3249

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World J Virology. Nov 12, 2015; 4(4): 365-371
Published online Nov 12, 2015. doi: 10.5501/wjv.v4.i4.365

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Figure 3 Agarose gel electrophoresis of different rotavirus genotype using reverse transcriptase polymerase chain reaction. Amplicon sizes for different genotypes (G1: 618 bp; G2: 521 bp; G3: 682 bp; G4: 452 bp; G9: 179 bp; G12: 396 bp; P[4]: 362 bp; P[8]: 224 bp. M: 100 bp DNA ladder (life technologies).

Citation: Tong Y, Lee BE, Pang XL. Rapid genotyping of human rotavirus using SYBR green real-time reverse transcription-polymerase chain reaction with melting curve analysis. World J Virology 2015; 4(4): 365-371
URL: https://www.wjgnet.com/2220-3249/full/v4/i4/365.htm
DOI: https://dx.doi.org/10.5501/wjv.v4.i4.365