Copyright
©The Author(s) 2015.
World J Virology. Nov 12, 2015; 4(4): 323-342
Published online Nov 12, 2015. doi: 10.5501/wjv.v4.i4.323
Published online Nov 12, 2015. doi: 10.5501/wjv.v4.i4.323
Table 3 Performance characteristics of commercial methods for hepatitis B virus and hepatitis C virus detection and quantification
| Assay (manufacture) | Method | Measurable range (IU/mL) | Limit of detection IU/mL (using WHO HBV standard) | Conversion factor (IU/mL to copies/mL) |
| HBV | ||||
| Cobas ampliPrep/Cobas TaqMan HBV test v2.0 (Roche Diagnostics, California, United States) | Semi-automated qPCR | 20-1.7 × 107 | 20 | 5.82 |
| Cobas TaqMan HBV test (for use with high pure system) | Semi-automated real time PCR | 29-1.1 × 107 | 6 | 5.82 |
| Abbott real time HBV (Abbott Diagnostic, Chicago, United States) | Automated real time PCR | 10-1 × 109 | 10 | 3.41 |
| Versant HBV 3.0 assay (Siemens Healthcare, United States) | Branched DNA | 2000-1 × 108 | 2000 | 5.6 |
| HCV qualitative | ||||
| Cobas Amplicor HCV v2.0 (Roche) | Semiautomated RT-PCR | 50 | 50 | - |
| Versant HCV RNA qualitative assay (TMA) | Transcription-mediated amplification | < 9.6 | 5-9.6 | - |
| Quantitative | ||||
| Versant HCV RNA 3.0 (Siemens) | Branched DNA | 7.7 × 106 | 615 | 5.2 |
| Cobas Amplicor Monitor HCV v2.0 (Roche) | Semiautomated RT-PCR | 5 × 105 | 600 | 2.7 |
| Real time HCV (Abbott) | Real time PCR | 1 × 107 | 10 | 3.8 |
| Cobas AmpliPrep/Cobas TaqMan (Roche) | Automated real time PCR | 6.9 × 107 | 43 | 3 |
| High Pure/Cobas TaqMan (Roche) | Semiautomated real time PCR | 3.9 × 108 | 25 | 3 |
- Citation: Villar LM, Cruz HM, Barbosa JR, Bezerra CS, Portilho MM, Scalioni LP. Update on hepatitis B and C virus diagnosis. World J Virology 2015; 4(4): 323-342
- URL: https://www.wjgnet.com/2220-3249/full/v4/i4/323.htm
- DOI: https://dx.doi.org/10.5501/wjv.v4.i4.323