In vitro intracellular IFNγ, IL-17 and IL-10 producing T cells correlates with the occurrence of post-transplant opportunistic infection in liver and kidney recipients

Figure 1 FACS analysis of cultured CD3⁺ T lymphocyte. Whole blood peripheral CD4⁺ and CD8⁺ T lymphocytes from individual ESLF and ESRF patients as well as HC subjects were stain with anti-CD3, anti-CD8, anti-CD69, anti-IL-17, anti-IL-10 and anti-IFNγ monoclonal antibodies following manufacture’s guidelines upon in vitro stimulation with Io and PMA. CD4⁺ and CD8⁺ lymphocytes were gated within CD3⁺CD69⁺ population following polyclonal activation for 4 h. CD4⁺ T cells were consider to approximate CD3⁺CD8^- T cells. At least 50000 events were acquired. Io: Ionomycin; PMA: Phorbol myristate acetate.