Islet dimension and its impact on transplant outcome: A systematic review

Figure 4 Isolated human islets at various stages of assessment before transplantation. A: Unstained isolated human islets of different sizes (S: Small-sized islet; L: Large-sized islet); B: Dithizone (Diphenylthiocarbazone)-stained human islets, freshly isolated, showing the purity of the preparation; C: Unstained normal human islet under higher magnification; D: Unstained human islet with central necrosis after culture for 5 days, viewed under higher magnification; E: Haematoxylin and Eosin-stained image of large-sized islet in native pancreas with multiple intra-islet capillaries (black arrowhead) (higher magnification); F: Fluorescein diacetate/propidium iodide staining to assess the viability of the isolated islets (Bright green fluorescence indicates live cells, while bright red or orange fluorescence indicates dead or necrotic cells).