Pathophysiology of acute graft-versus-host disease from the perspective of hemodynamics determined by dielectric analysis

Figure 5 We conducted a replacement experiment for the blood cells and plasma components. A: Blood sample (about 30%Hct) from the patient with graft-versus-host disease was diluted as 10%Hct diluted in phosphate buffer saline (PBS), 10%Hct diluted in the patient’s plasma, 10%Hct replaced in PBS, and 10%Hct replaced in PBS with 10%FBS, and dielectric properties were measured; B: (I) Blood sample (about 30%Hct) from the patient with graft-versus-host disease was diluted as (1) 10%Hct diluted in PBS, (2) 10%Hct diluted in the patient’s plasma, (3) 10%Hct replaced in PBS, and (4) 10%Hct replaced in PBS with 10%FBS, and observed by phase-contrast microscopy; (II) Sample of (I) was further diluted by 50-fold in the same way, and observed by phase-contrast microscopy. PBS: Phosphate buffer saline.