Electroacupuncture alleviates depression and gastrointestinal dysfunction by rebalancing GABAergic activity in the central amygdala

Figure 2 Electroacupuncture alleviates gastric dysfunction in chronic unpredictable mild stress mice. A: Experimental timeline showing model preparation, electroacupuncture, behavioral testing, and gastric assessments; B: Representative three-dimensional heatmap of free-feeding behavior; C: Quantification of percentage of time spent in the feeding zone (free-feeding test); D: Average body weight changes over 28 days; E: Average 1-hour food intake over 28 days; F: Representative trace of gastric motility; G: Quantification of average periodic amplitude of gastric contractions; H: Quantification of mean gastric motility frequency; I: Representative fluorescent in vivo imaging of gastric emptying; J: Quantification of gastric emptying over 75 minutes (percent fluorescence loss relative to baseline at time 0). Data are mean ± SE; statistics by one-way or two-way analysis of variance as appropriate; n = 10 mice in each group. ^bP < 0.01, ^cP < 0.001. CUMS: Chronic unpredictable mild stress; EA: Electroacupuncture.