Sini-Suanzaoren decoction regulates mitochondrial biogenesis mediated by MT-SIRT1 in the treatment of insomnia rats

Figure 5 Immunohistochemical, PCR, and Western blot results of rats in each group. A: Immunohistochemical results; B-G: PCR detection of the mRNA expression of AANAT, SIRT1, PGC-1α, NRF1, TFAM and mtDNA in the hypothalamus; H: Western blot detection of the protein expression of AANAT, SIRT1, PGC-1α, NRF1, TFAM in the hypothalamus. ^aP < 0.001, model group vs control group; ^bP < 0.001, Sini-Suanzaoren decoction (SNSZRD) high dose group vs model group; ^cP < 0.001, SNSZRD middle dose group vs model group; ^dP < 0.01, positive group vs model group; ^eP < 0.01, SNSZRD middle dose group vs model group; ^fP < 0.05, SNSZRD low dose group vs model group; ^gP < 0.05, SNSZRD middle dose group vs model group.