Sini-Suanzaoren decoction regulates mitochondrial biogenesis mediated by MT-SIRT1 in the treatment of insomnia rats

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World Journal of Psychiatry

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2220-3206

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Psychiatry. Dec 19, 2025; 15(12): 108867
Published online Dec 19, 2025. doi: 10.5498/wjp.v15.i12.108867

Table 1 Primer sequence of internal reference gene in quantitative real-time PCR

Gene	Forward primer (5′-3′)	Reverse primer (5′-3′)	Product length (bp)
AANAT	CCACCAGTGCGTTTGAGATT	GACACAGGGTGAGGAAGTGC	99
SIRT1	AGGGAACCTCTGCCTCATCT	TGGCATACTCGCCACCTAAC	100
PGC1	GTGCAGCCAAGACTCTGTATGG	GTCCAGGTCATTCACATCAAGTTC	121
NRF1	TATGGCGGAAGTAATGAAAGACG	CAACGTAAGCTCTGCCTTGTT	101
TFAM	ATCAAGACTGTGCGTGCATC	AAAGCCCGGAAGGTTCTTAG	217
GAPDH	CTGGAGAAACCTGCCAAGTATG	GGTGGAAGAATGGGAGTTGCT	138
Cyb	ATCTCACCCCCTATTCAAAATCATC	GGTATCAGACGTGTAGTGTATTGC	160

Citation: Li RT, Lan BJ, Xiao ZY, Shi QH, Chen XY, Li F. Sini-Suanzaoren decoction regulates mitochondrial biogenesis mediated by MT-SIRT1 in the treatment of insomnia rats. World J Psychiatry 2025; 15(12): 108867
URL: https://www.wjgnet.com/2220-3206/full/v15/i12/108867.htm
DOI: https://dx.doi.org/10.5498/wjp.v15.i12.108867

Li RT, Lan BJ, Xiao ZY, Shi QH, Chen XY, Li F. Sini-Suanzaoren decoction regulates mitochondrial biogenesis mediated by MT-SIRT1 in the treatment of insomnia rats. World J Psychiatry 2025; 15(12): 108867 [PMID: 41357936 DOI: 10.5498/wjp.v15.i12.108867]

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