Exosomal miR-320e through wnt2targeted inhibition of the Wnt/β-catenin pathway allevisate cerebral small vessel disease and cognitive impairment

Figure 5 The expression of miR-320e in the exosomes secreted by the miR-320e mimic-transfected cells was significantly higher than that in the mimic NC group and the blank control. A: HUVECs were transfected using miR-320e mimics and the expression of miR-320e was detected. B: Lipid membrane of HUVECs marked by DiI; C: Microplate reader analysis showed that exosomes were also marked by DiI; D: As shown by using marked exosomes harvested from HUVECs and cocultured with HASMCs, HUVECs also showed red fluorescence; E-G: Western blotting and PCR showed that Wnt2 and β-catenin expression in the Exo-mimic group decreased compared to that of the control group. HUVECs: Human umbilical vein endothelial cells; HASMCs: Human aortic vascular smooth muscle cells; PCR: Polymerase chain reaction. ^aP < 0.05, ^bP < 0.01.