Synthetic messenger RNA vaccines and transcriptomic dysregulation: Evidence from new-onset adverse events and cancers post-vaccination

Figure 1 Transcriptome-wide differential expression profiles in vaccine-affected individuals. A: Volcano plot showing differential gene expression in individuals with new-onset adverse events following messenger RNA (mRNA) coronavirus disease 2019 (COVID-19) vaccination (n = 3) compared to normal controls (n = 803); B: Volcano plot showing differential gene expression in individuals diagnosed with new-onset cancer shortly after receiving mRNA COVID-19 vaccination (n = 7) compared to the same control cohort. Each point represents a single gene plotted by log₂ fold change (X-axis) and -log₁₀ adjusted P value (P_adj) (Y-axis). Genes with significant upregulation (log₂FC > 1, P_adj < 0.05) are marked in red, while significantly downregulated genes (log₂FC < -1, P_adj < 0.05) are shown in blue. Non-significant genes appear in gray. These plots reveal widespread transcriptional dysregulation in both patient groups, serving as the foundation for subsequent pathway enrichment analysis.