Proneurogenic and microglial modulatory properties of botulinum neurotoxin in the hippocampus of aging experimental mice

Figure 2 Confocal microscopy analysis of bromodeoxyuridine - neuronal nuclei positive neurons in the hippocampal dentate gyrus. A: Representative confocal Z-scan image showing a bromodeoxyuridine (BrdU)-neuronal nuclei (NeuN) double-positive neuron in the hippocampal dentate gyrus (DG) region of experimental mice. Scale bar = 50 μm; B: Bar graph representing the percentage of BrdU-NeuN positive newborn neurons in the hippocampal DG. A significant increase in the percentage of BrdU-NeuN positive neurons was observed in the botulinum neurotoxin group compared to the control group (^bP value ≤ 0.01). DG: Dentate gyrus; BrdU: Bromodeoxyuridine; NeuN: Neuronal nuclei.