Chemical profiling of bioactive compounds in the methanolic extract of wild leaf and callus of Vitex negundo using gas chromatography-mass spectrometry

Table 1 Effect of combinations of Plant Genetic Resources in mass spectrometry medium on callus induction in Vitex negundo

MS + hormone (mg/L)	Medium name	Source of explants	Number of explants inoculated	Callus response (%)	Callus genesis	Callus initiation (time/d)	Color	Texture
MS + no hormone	M1	Leaf	13	-	-	-	-	-
MS + BAP (2.0) + 2,4D (0.2)	M2	Leaf	13	15 d	+++	100%	Green	Friable
MS + BAP (2.0) + NAA (0.2)	M3	Leaf	13	15 d	++	70%	Greenish white	Friable
MS + DPU (2.0) + 2,4D (0.2)	M4	Leaf	13	15 d	+++	90%	White-yellowish	Loose
MS + DPU (2.0) + NAA (0.2)	M5	Leaf	13	15 d	+	40%	White-yellowish	Loose

MS: Mass spectrometry; BAP: 6-benzylaminopurine; NAA: α-naphthylacetic acid; DPU: Di-phenylurea.