Barriers in contribution of human mesenchymal stem cells to murine muscle regeneration

Figure 5 Myoregeneration kinetics of minced muscle implants without and with mesenchymal stem cells. A: The data represent visual estimates derived from microscopic examination of 2 or 3 desmin-stained sections through the center of fresh (square frame and solid line) and cryopreserved (triangle and broken line) implants. Each point represents the mean with SD of between 2 and 10 (average 4) implants; B: Comparison between visual estimation and electronic calculation of areas of myoregeneration. Thirteen desmin-stained cross sections were analyzed by both visual estimation (plotted on the Y-axis) and with the Interactive Image Measurement program of CELL^F imaging software (plotted on the X-axis). The excellent correlation between the two methods of analysis justifies the choice of the less laborious visual estimation procedure.