Internal ribosome entry site-based vectors for combined gene therapy

Table 1 Preclinical studies of combined gene therapy with co-administration of monocistronic vectors

Pathology	Therapeutic genes	Animal model	Vector type	Ref.
Cancers
Leukemia, melanoma	Angiostatin + endostatin	Mouse	Retrovirus	Scappaticci et al[4], 2001
Ovarian cancer	Angiostatin + endostatin	Mouse	AAV	Ponnazhagan et al[3], 2004
Glioblastoma	VEGF-R1 + angio-endo (Statin AE)	Mouse	SB transposon	Ohlfest et al[60], 2005
Pancreatic cancer	TSP1 + endostatin	Mouse	AAV	Zhang et al[61], 2007
Cardiovascular diseases
Limb ischemia	VEGFA + angiopoietin-1	Rabbit	Plasmid	Chae et al[5], 2000
Limb ischemia	VEGFA + FGF2	Mouse	Plasmid	Lee et al[59], 2007
Limb ischemia	VEGFA + PDGFB	Rabbit	AAV	Kupatt et al[58], 2010
Heart ischemia	VEGFA + PDGFB	Pig	AAV	Kupatt et al[58], 2010
Rear diseases
DMD	Microdystrophin + IGF1	Mouse	AAV	Abmayr et al[62], 2005

DMD: Duchenne muscular dystrophy; VEGF: Vascular endothelial growth factor; FGF2: Fibroblast growth factor 2; AAV: Adeno-associated virus.