Epigallocatechin-3-gallate suppresses transforming growth factor-beta signaling by interacting with the transforming growth factor-beta type II receptor

Figure 4 (-)-Epigallocatechin-3-gallate interferes with binding between transforming growth factor-β and its type II receptor. A: Positive control of the immunoprecipitation experiment. Cell lysates from transfected COS-7 cells were treated with anti-transforming growth factor-β type II receptor (TGFRII). TGFRII was recovered in the immunoprecipitation product of the lysate; B: Effects of (-)-epigallocatechin-3-gallate (EGCG) and catechin on the antigen-antibody interaction. After cells were treated with EGCG or catechin, anti-TGFRII bound to Protein G was added to each lysate. Western blotting was performed using anti-TGFRII.