Quantitative assessment of STAT3 and HPV16 E6 transcripts using Flow-FISH approach for early detection of progressive cervical lesions

Figure 3 Estimation of specificity of signal transducer and activator of transcription 3 and human papillomavirus 16 E6 at different probe concentrations. ^aP < 0.05 vs C33a scrambled, ^bP < 0.0001 vs SiHa scrambled. A: Representative fluorescence photomicrographs showing expression of signal transducer and activator of transcription 3 (STAT3) transcripts in C33a and SiHa at different concentrations of the probe. The upper panel represent the signal intensity at 0.1 μmol/L probe concentration and lower panel represent the signal intensity at 0.2 μmol/L probe concentration. Non-specific scrambled probe was used as a negative control to estimate the label of non-specific binding of the probe (scale bar: 20 μm). Bar graph showing fluorescence intensity of STAT3 probes and STAT3 scrambled probes; B: Representative fluorescence photomicrographs showing expression of human papillomavirus (HPV) 16 E6E7 transcripts in SiHa (scale bar: 20 μm). HPV16E6 scrambled probe was used as a negative control to estimate the specificity of the probe. Bar graph showing fluorescence intensity of HPV16 E6 probes and HPV16 E6 scrambled probes in SiHa. HPV: Human papillomavirus; STAT3: Signal transducer and activator of transcription 3; SCR: Single locus scrambled probes.