Quantitative assessment of STAT3 and HPV16 E6 transcripts using Flow-FISH approach for early detection of progressive cervical lesions

Figure 1 Analysis of human papillomavirus and signal transducer and activator of transcription 3 transcripts in cervical cancer cell line using real-time reverse transcription-polymerase chain reaction. ¹Spliced variant of E6. A: Representative gel images showing amplicons of reverse transcriptase-glyceraldehyde-3-phosphate dehydrogenase; B: RT-human papillomavirus (HPV) 16 E6; C: RT-HPV16 E7; D: RT-signal transducer and activator of transcription 3; E: RT-HPV18 E6. In addition to the full-length HPV18 E6 transcript, a 245 bp amplicon corresponding to the spliced variant HPV18 E6 was observed; F: RT-HPV18 E7. RT-PCR: Real-time reverse transcription-polymerase chain reaction; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; HPV: Human papillomavirus; STAT3: Signal transducer and activator of transcription 3.