Human ciliary muscle cell responses to kinins: Activation of ERK1/2 and pro-matrix metalloproteinases secretion

Figure 4 Extracellular signal-regulated kinase-1/2 activation in cultured primary human ciliary muscle cells. Effects of two B₂-receptor antagonists on kinin-induced ERK1/2 activation by three BK agonists are shown in (A). Effect of bromfenac on kinin-mediated ERK1/2 activation are shown in (B). Serum-deprived h-CM cells were incubated for 10 min with 100 nmol/L B₂ selective agonists BK, Met-Lys-BK and RMP-7. In experiments with antagonists or bromfenac, cells were pretreated with 100 nmol/L B2 receptor antagonist HOE-140 or WIN-64338 or bromfenac (1 μmol/L) for 15 min prior to addition of BK agonists. At the end of incubation, cell lysates were analyzed for phosphorylated ERK1/2 using Cellul’erk kit from CisBio. Significantly different than basal responses, ^aP < 0.05 by Student’s t-test. BK: Bradykinin; ERK1/2: Extracellular signal-regulated kinase-1/2; h-CM: Human ciliary muscle; BF: Bromfenac.