Human ciliary muscle cell responses to kinins: Activation of ERK1/2 and pro-matrix metalloproteinases secretion

Figure 3 Effect of B₂ and B₁ selective agonists on extracellular signal-regulated kinase-1/2 activation in cultured primary human ciliary muscle cells. Serum-deprived h-CM cells were incubated for 10 min with 100 nmol/L B₂ selective agonists (BK, Met-Lys-BK and RMP-7) or B₁ selective agonist (Des-Arg⁹-BK). At the end of incubation, cell lysates were analyzed for phosphorylated ERK1/2 using Cellul’erk kit from CisBio. Differs from base-line control, ^aP < 0.05 by Student’s t-test. ERK1/2: Extracellular signal-regulated kinase-1/2; h-CM: Human ciliary muscle; BK: Bradykinin.