Hypoxia and cytokines regulate carbonic anhydrase 9 expression in hepatocellular carcinoma cells in vitro

Figure 2 Western blotting analysis of hypoxia-inducible factor-1 alpha protein expression under hypoxia or cytokine stimulation (interleukin-1, interleukin-6, tumor necrosis factor-alpha or transforming growth factor-beta) in nuclear extracts of the hepatocellular carcinoma cell line Hep3B. Treatment with 100 μmol/L DFO under aerobic conditions served as a positive control. One representative Western blotting of hypoxia-inducible factor-1 alpha (HIF-1α) protein with β-tubulin as a loading control and quantification of the results via densitometry. IL: Interleukin; TNF-α: Tumor necrosis factor-alpha; TGF-β: Transforming growth factor-beta.