Hypoxia and cytokines regulate carbonic anhydrase 9 expression in hepatocellular carcinoma cells in vitro

Figure 1 Hypoxia-inducible factor-1 alpha mRNA expression level determination via semi-quantitative reverse transcription polymerase chain reaction in the human hepatocellular carcinoma cell line Hep3B under aerobic and hypoxic conditions (0. 1% O₂ for 24 h) without or with cytokine stimulation (interleukin-1, interleukin-6, tumor necrosis factor-alpha or transforming growth factor-beta) under both aeration conditions examined. β-actin was used as a loading control. Bar graphs show band intensities after densitometric evaluation. Representative experiment of three different experiments. IL: Interleukin; TNF-α: Tumor necrosis factor-alpha; TGF-β: Transforming growth factor-beta; HIF-1α: Hypoxia-inducible factor-1 alpha.