Overexpression of low-molecular-weight caldesmon is associated with aggressive phenotypes and epithelial-mesenchymal transition in breast cancer cells

Figure 4 Functional consequences of CALD1 silencing on invasion in CAL-51 breast cancer cells. CALD1 silencing significantly reduced collagen invasiveness in the metastatic CAL-51 cell line, as assessed by an invasion assay. A: Representative images showing high invasive capacity of untransfected CAL-51 cells, characterized by a large number of invading cells; B: Marked reduction in invasive cells following CALD1 silencing in CAL-51 cells (small interfering RNA-CALD1); C: Cells transfected with negative control small interfering RNA exhibited invasive characteristics similar to untransfected CAL-51 cells (× 100 magnification); D: Quantitative analysis of invasion measured by absorbance at 492 nm. ^aP < 0.0080. N.C: Negative control small interfering RNA; siRNA: Small interfering RNA.