Overexpression of low-molecular-weight caldesmon is associated with aggressive phenotypes and epithelial-mesenchymal transition in breast cancer cells

Figure 1 Differential expression of the low-molecular-weight isoform of caldesmon in breast cancer cell lines. Western blot analysis of the low-molecular-weight isoform of caldesmon (l-CAD) expression in breast cancer cell lines is shown. A: Representative western blot results showing the expression of l-CAD in breast cancer cell line MCF7 and its isogenic derivative 1001, ZR-75-1, CAL-51, MDA-MB-231, BT-549, MDA-MB-361, and T47D cell lines. HeLa cells were used as a positive control. β-actin was used as a loading control; B: Densitometric quantification of l-CAD band intensity normalized to b-actin, presented as a bar graph. l-CAD: Low-molecular-weight isoform of caldesmon.