Gene and cytokine expression profiles of metastatic colorectal cancer patients post reovirus administration

Figure 3 Alterations of GSK3β expression are correlated with alterations in interferon gamma, interleukin-8, regulated upon activation, normal T-cell expressed and secreted, and interleukin-6 expression. A: The relative change GSK3β (by delta-delta cycle three hold calculations) over time. Expression of the GSK3β gene was significantly upregulated on day 2 (P = 0.03) and significantly downregulated on days 8 (P = 0.008) and 15 (P = 0.03). Error bars represent one standard error of the mean of the data; B: The relative changes in interferon gamma (IFN-γ), interleukin (IL)-8, regulated upon activation, normal T-cell expressed and secreted (RANTES), and IL-6 expression (by mean expression across all reovirus-treated patients) over time. Statistically significant increases were observed for RANTES on day 2 (P = 0.04); IL-6 on day 8 (P = 0.0001); and IFN-γ on day 15 (0.0004). Statistically significant decreases were observed for RANTES on day 8 (P = 0.02); IFN-γ on day 3 (P = 0.0001); and IL-8 on days 3 (P = 0.02), 4 (P = 0.00003), and 15 (P = 0.03). Error bars represent one standard of mean of the data. IFN: Interferon; IL: Interleukin; RANTES: Regulated upon activation, normal T-cell expressed and secreted.